Ex situ Voltammetry and Chronopotentiometry of Doxorubicin at a Pyrolytic Graphite Electrode: Redox and Catalytic Properties and Analytical Applications
Ex situ (adsorptive transfer stripping) electrochemical techniques in connection with basal-plane PGE have been applied to the study of redox and catalytic properties of doxorubicin (DOX). Cyclic and square-wave voltammetry and constant current chronopotentiometric stripping (CPS) analysis were used to follow reversible reduction of DOX quinone moiety around -0.5 V and its Coupling to catalytic oxygen reduction. CPS was for the first time used for sensitive ex situ determination of the DOX using the catalytic signal around - 0.5 V in the presence of oxygen, allowing detection of femtomole amounts of DOX. We show that specific interaction of DOX with double-stranded DNA can easily be monitored using the catalytic CPS signal.