Vybavení
- Hypoxbox Invivo2 400 (Baker Ruskinn) for cell culturing in hypoxia
- PCR thermocyclers: PTC-100 (MJ Research) and some others. Applications: FISH probe denaturation, genome fragment amplification, colony screening, plant genotyping, preparation and control of gene constructs
- 4D-Nucleofector (Lonza) – a transfection device for cells that can be transfected with difficulty
- Flowcytometer Muse Cell Analyzer (Merck Millipore): Analyses of cell numbers, cell cycle distribution, oxidative stress, DNA damage (various approaches), cell viability and apoptosis
- xCELLigence RTCA DP (ACEA Biosciences); for label-free, real-time, automated monitoring of cell status in a variety of cell-based assays. Real time measurements of cell proliferation and migration
- Quantitative Real-Time PCR: QuantStudio5 (Applied Biosystems): quantification of RNA levels of genes of interest
- Fluorescence microscope Leica DMIL LED (10, 40, and 100x lenses) and other laboratory microscopes: cell culture observation and documentation, transfected clones selection, etc.
- Western blot system for wet/semi-dry transfer: quantification of protein levels
- NanoQUANT Infinite M200 Pro Plate Reader (Tecan): Spektrofotometer for plate absorption measurements in visible light (can be upgraded for fluorescence module)
- Nanodrop: UV spektrofotometr for sample concentration measurements in very small volumes
- Gel electrophoresis PAGE, agarose gels, SDS-PAGE. Applications: DNA, RNA, and protein analyses
- Hemocytometer (Mindray BC-5300 Vet) – hematology Analyzer with differential analysis for human and animal samples
- Cell incubators (thermostats with CO2 atmosphere) for cell culturing (especially human normal and tumor cell lines, potentially bacteria)
- Flow-boxes for sterile work
- RNA laboratory
- Laboratory for open radiation sources work
- Laboratory for mice autopsy
- Chamber for plant cultivation (TBY-2, Arabidopsis, green algae)
- Hypoxic chamber for cell culturing under adjustable hypoxic conditions
- Techniques of telomere biology: TRAP for telomerase activity and TRF for telomere length measurements
- Spektrofluorometr FlouroMax+ (fy Horiba): quantitative measurements of DNA-protein and protein-protein interactions, mainly based on fluorescence anisotropy. Also, fluorescence lifetime measurements of biologically important complexes.
- Camera with transiluminator (Basler AG, Germany): For cell colonies counting and image processing.
- Thermostat 20 (Sanyo): for plant tissue cultures
- Flowbox (FluFRANCE): for plant tissue cultures
In share:
- Chisostat (Chirana): gamma ray-irradiator (with a 60Co source): animal and cell/other sample irradiation, devices sterilization
- Leica DMRXA fluorescence microscope equipped with a spinning disks and lasers for 3-colour confocal microscopy. Most frequently used labelling techniques, immunolabeling, FISH, immuno-FISH, GFP protein spectral variants-tagging technique.
- Leica SP5 microscopy system with a box for living cell microscopy (temperature, CO2), white laser for multi-color labelling and a powerful laser for cell micro-irradiation (ability of localized DNA damage induction at the microscale).
- Single Molecule Localization Microscopy (SMLM) – access in the frame of cooperation with Kirchhoff Institute for Physics, Heidelberg University, Heidelberg, Germany)
Software
- STORM deconvolution software for superresolution
- DeepFoci – Software created by us (Tomáš Vičar, Ph.D.) for the detection, quantification and analysis of DSB repair foci (IRIF) at the micro level. The software is based on artificial neural networks and deep machine learning, and can therefore be trained for gH2AX and specific repair proteins, and specific experimental and microscopy conditions.
Other routine equipment: centrifuges, rollers, scales, thermoblock, shakers, autoclave, pH meter, fume cupboard/hood)