Overview

AB Attune:

Flow cytometer equipped with two lasers (405 and 488nm) with separate optical paths and six detectors with high resolution (4.194.304 channels). The instrument uses a unique technology of acoustic focusing of the sample, which enables the user to analyse big sample volume in a short time (up to 1mL/min) and detect rare populations in your sample. The cytometer is controlled by the Attune Cytometric Software application, which is installed on a powerful workstation.

BD FACSAria II Sorp:

Flow cytometer and 4-way cell sorter equipped with five independently disconnectable lasers (355nm, 405nm, 488nm, 561nm and 639nm) with separated optical paths and fix optics. It uses the principle of hydrodynamic focusing in the environment of high pressure. The instrument can measure FSC, SSC and 18 fluorescence parameters at the same time and also aseptically sort particles up to 4 tubes or multiple-well plate (up to 384-wells), these can be cooled or heated on preset temperature by the connected water bath. The cytometer contains digital electronics, which can detect 70.000 events/sec. in 262.144 channels and sort the particles up to 20 μm size at speed of 40.000 events/sec. in 1.024 channels resolution. BD FACSDiva software is used for a preset of sorting, data acquisition and analysis.

BD FACSVerse:

Flow cytometer equipped with three lasers (405nm, 488nm and 640nm) with separated optical paths. The fluidics system uses vacuum, excitation of a sample takes place in the steel cuvette, which improves optical parameters. The instrument can measure FSC, SSC and 8 fluorescence parameters. The cytometer is fully digital and can analyse 35.000 events/sec in 262.144 channels. It contains automatic universal sample loader, which helps automatically analyse great volumes of samples (up to 40 tubes), sample can be aspirated not only from tube, but also from multi-well plate (up to 384-wells). Advanced BD FACSSuite software is used for a data acquisition and analysis, software automatically recalculates compensation of spillover of individual fluorochromes on detectors after fluorochrome implementation, so that user can change the sensitivity of detectors at will.

SONY SP6800:

Spectral analyzer equipped with three lasers (405, 488nm and 638nm) which is able to detect also dim fluorescence signals from single particles in complete range from 420 to 800nm with using 32 PMT detectors. Fully digital cytometer with speed of analysis 20.000 events/sec. The machine simplifies detection of multicolour experiments (more than 16 colours), and helps to discriminate signal from fluorochromes with very similar spectra. Emission of fluorochromes/antibodies is saved to spectral library. Next, the software uses algorithm for spectral unmixing and the user is able to differentiate signals from separate fluorochromes visualized on standard dot plots together with extracted influence of cell autofluorescence. PC station is equipped with software for quantification of fluorescence spectra FCS Express (De Novo Software).

BD FACSDiscover S8 with CellView:

Spectral flow cytometer capable of up to six-way sorting. It is equipped with a total of five lasers (349nm, 405nm, 488nm, 561nm, 637nm), with a separate array of detectors for each laser, for a total of 78 detectors covering a spectrum from 365 to 860nm. The spectral cytometer is capable of analyzing up to 30,000 particles/s. It is equipped to perform large-scale multi-color experiments (more than 40 colors) and is also capable of resolving fluorochromes with similar emission spectra. The stored emission spectra for individual fluorochromes are then used for so-called spectral unmixing, which can be used to resolve individual fluorochromes. The system is capable of resolving up to 100 million intensities. The instrument is equipped to detect FSC and SSC signals using both a 488nm laser and a 405nm laser. This cytometer is equipped with BD's unique CellView technology, which allows images of cells including up to four fluorescence signals to be captured, and individual cell sorting can then be performed based on these images. BD FACSChorus software is used to set up analysis, sorting and data evaluation.

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