Adsorption/desorption behavior of hyaluronic acid fragments at charged hydrophobic surface

Journal: Carbohydrate Polymers
Authors: Švecová E., Ostatná V., Fojt L., Hermannová M., Velebný V., Ondreáš F.
Year: 2022
ISBN: 0144-8617


This work reveals the growing potential of novel electrochemical methods that are applicable for poly- saccharides. It was shown for the first time that the molecules of hyaluronic acid (HA) exhibit electrochemical response using phase-sensitive alternating current (AC) voltammetry in phase-out mode. Adsorption and desorption processes of HA fragments at a charged interface of mercury electrode were observed in buffered HA solutions. Electrostatic and hydrophobic manners of interactions were distinguished for native hyaluronan fragments in a wide electric potential range. The AC voltammetry response depended on the temperature, concentration, and length of HA chains. Results of this work open possibilities for further structural character- ization of widely used HA fragments and understanding manners of interactions with charged hydrophobic surfaces that could be useful in the future for understanding HA interactions at biological levels.

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