Hyršlová Vaculová Alena, Doc. RNDr., Ph.D.

Klapka: +420 541-517- 169
Email: vaculova@ibp.cz

Doc. RNDr. Alena Vaculova, Ph.D.
vaculova@ibp.cz
+420 541517169

 

Education:

B.Sc. - 1998 - General Biology, Faculty of Science, MasarykUniversity, Brno, CR

M.Sc. - 2000 - Animal Physiology, Faculty of Science, MasarykUniversity, Brno, CR

Ph.D., RNDr. – 2003, 2004 - Animal Physiology, Faculty of Science, MasarykUniversity, Brno, CR

Current position:

Associate Scientist – Department of Cytokinetics, Instituteof Biophysics, Academyof Sciencesof the Czech Republic, v.v.i., Brno, Czech Republic(since 2009)

 

Previous positions:

Two–year scientific stay – Toxicology, Instituteof Environmental Medicine, Karolinska Institutet, Stockholm, Sweden(2007 - 2009)

Research Scientist – Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic (2003-2007)

 

Awards:

1999 – Award of the Department of Biology, Faculty of Science, Masaryk University Brno, for excellent study results.

2004 – Award of the Instituteof Biophysics, Academyof Sciencesof the CzechRepublic, for excellent scientific results in 2004.

2005 – Poster first price, international conference on Analytical Cytometry, Červenohorské sedlo, CzechRepublic, 2005.

 

Professional Memberships:

European Cell Death Organization;FEBS Constituent Society (Czech Society for Biochemistry and Molecular Biology); Czech Society for Analytical Cytometry

 

Teaching activities:

External teacher at Faculty of Sciences, MasarykUniversity, Brno, CR. Courses: Methodology of Scientific Work, Analytical Cytometry, Molecular Physiology of Animals, Mechanisms of Cell Death, Function, Methods.Supervisor of undergraduate and Ph.D. students (MU, Brno, CR). Member of examination committee for postgraduate students - Animal Physiology, MU Brno.

 

Research Interests:

cancer cell death, molecular mechanisms of apoptosis induced by TRAIL and chemotherapy in cancer cells, mechanisms of cancer cell resistance and sensitization to TRAIL, cancer biology

 

Publications:

Svihálková-Sindlerová L, Foltinová V, Vaculová A, Horváth V, Soucek K, Sova P, Hofmanová J, Kozubík A. (2010). LA-12 overcomes confluence-dependent resistance of HT-29 colon cancer cells to Pt (II) compounds. Anticancer Res30(4):1183-8.

Vaculova A., Kaminskyy V., Jalalvand E., Surova O., Zhivotovsky B. (2010) Doxorubicin and etoposide sensitize small cell lung carcinoma cells expressing caspase-8 to TRAIL. Mol Cancer9:87-99.

Sundström JF1, Vaculova A1, Smertenko AP1, Savenkov EI1, Golovko A1, Minina E1, Tiwari BS, Rodriguez-Nieto S, Zamyatnin AA Jr, Välineva T, Saarikettu J, Frilander MJ, Suarez MF, Zavialov A, Ståhl U, Hussey PJ, Silvennoinen O, Sundberg E, Zhivotovsky B, Bozhkov PV. (2009). Tudor staphylococcal nuclease is an evolutionarily conserved component of the programmed cell death degradome. Nature Cell Biology, 11, 1347-54. (1authors contributed equally).

Vaculová, A., Hofmanová, J., Zatloukalová, J., Kozubík, A. (2009). Differences in TRAIL-induced changes of Mcl-1 expression among distinct human colon epithelial cell lines. Exp Cell Res 315, 3259-66.

Hofmanová, J., Vaculová, A., Koubková, Z., Hýzd'alová, M., Kozubík, A. (2009). Human fetal colon cells and colon cancer cells respond differently to butyrate and PUFAs. Mol Nutr Food Res., 53 Suppl 1:S102-13.

Hofmanova, J., Vaculova, A., Hyzd'alova, M., Kozubik, A., (2008). Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers. Oncol Rep. 19, 567-73.

Hyzd'alova, M., Hofmanova, J., Pachernik, J., Vaculova, A., Kozubik, A., (2008). The interaction of butyrate with TNF-alpha during differentiation and apoptosis of colon epithelial cells: Role of NF-kappaB activation. Cytokine, 44, 33-43.

Mikes, J., Kleban, J., Sackova, V., Horvath, V., Jamborova, E., Vaculova, A., Kozubik, A., Hofmanova, J., Fedorocko, P., (2007). Necrosis predominates in the cell death of human colon adenocarcinoma HT-29 cells treated under variable conditions of photodynamic therapy with hypericin. Photochem Photobiol Sci. 6, 758-66.

Vaculova, A., Hofmanova, J., Soucek, K., Kozubik, A., (2006). Different modulation of TRAIL-induced apoptosis by inhibition of pro-survival pathways in TRAIL-sensitive and TRAIL-resistant colon cancer cells. FEBS Lett. 580, 6565-9.

Hofmanova, J., Vaculova, A., Kozubik, A., (2005). Polyunsaturated fatty acids sensitize human colon adenocarcinoma HT-29 cells to death receptor-mediated apoptosis. Cancer Lett. 218, 33-41.

Hofmanova, J., Vaculova, A., Lojek, A., Kozubik, A., (2005). Interaction of polyunsaturated fatty acids and sodium butyrate during apoptosis in HT-29 human colon adenocarcinoma cells. Eur J Nutr. 44, 40-51.

Vaculova, A., Hofmanova, J., Andera, L., Kozubik, A., (2005). TRAIL and docosahexaenoic acid cooperate to induce HT-29 colon cancer cell death. Cancer Lett. 229, 43-8.

Hofmanova, J., Zadak, Z., Hyspler, R., Mikeska, J., Zdansky, P., Vaculova, A., Netikova, J., Kozubik, A., (2005). The effects of parenteral lipid emulsions on cancer and normal human colon epithelial cells in vitro. Physiol Res. 54, 409-18.

Vaculova, A., Hofmanova, J., Soucek, K., Andera, L., Kozubik, A., (2004). Ethanol acts as a potent agent sensitizing colon cancer cells to the TRAIL-induced apoptosis. FEBS Lett. 577, 309-13.

Vaculova, A., Hofmanova, J., Soucek, K., Kovarikova, M., Kozubik, A., (2002). Tumor necrosis factor-alpha induces apoptosis associated with poly(ADP-ribose)polymerase cleavage in HT-29 colon cancer cells. Anticancer Res. 22, 1635-9.

 

REVIEWS:

Vaculova, A., Zhivotovsky, B., (2008). Caspases: determination of their activities in apoptotic cells. Methods Enzymol. 442, 157-81.

Kozubik, A., Vaculova, A., Soucek, K., Vondracek, J., Turanek, J., Hofmanova, J., (2008). Novel Anticancer Platinum(IV)Complexes with Adamantylamine: Their Efficiency and Innovative Chemotherapy Strategies Modifying Lipid Metabolism. Met Based Drugs. 2008, 417897.

 

BOOK CHAPTERS:

Hofmanova J., Vaculova A., Hyzdalova M., Kozubik A.: Different response of normal and cancer human colon epithelial cells to dietary fatty acids and endogenous apoptotic regulators of TNF family, New Cell Apoptosis Research, Nova Science Publishers, 2007.

Hofmanova, J., Soucek, K., Vaculova A., Kozubik A.: Fatty acids in modulation of reactive oxygen species balance in cancer. Oxidants in Biology - A Question of Balance. Edited by Valacchi, G; Davis, PA., Springer, 2008.