Vítězslav Bryja, M.Sc., PhD.

Education:
1995-2000 – M.Sc. in Molecular Biology and Genetics; Faculty of Sciences, MasarykUniversity, Brno, Czech Republic
2000-2004 - PhD. in Neurosciences; 1^st Faculty of Medicine, CharlesUniversity, Prague, Czech Republic

Professional experience:
7/2000 – 2/2002 – junior research scientist, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic
7/2000 – 6/2004 – research scientist, Centerof Cell Therapyand Tissue Replacement, CharlesUniversity, Prague
3/2002 – 6/2004 – research scientist, Instituteof Experimental Medicine, Academyof Sciencesof the CzechRepublic, Prague, Czech Republic
7/2004 – 6/2007 - postdoctoral fellow, Laboratory of Molecular Neurobiology, Department of Molecular Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden (supervisor : Prof. Ernest Arenas)
from 1/2007 – research scientist, Laboratory of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
from 3/2007 – research scientist, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

Major grants:
EMBO Installation Grant: Functional analysis of dishevelledome (2008-2010), principal investigator
GrantAgencyof the Academyof Sciencesof the CzechRepublic: Posttranslational modification of Dishevelled (2008-2010), principal investigator
The Swedish Foundation for International Cooperation in Research and Higher Education (STINT): Frizzled signaling – protein-protein interactions and intracellular scaffolding as a means of signal transduction (2008-2011), main foreign partner.
Czech Science Foundation : Intercellular signalling in development and disease (2009-2012), main co-ordinator.
Czech Science Foundation : The role of beta-arrestin in achieving Wnt signalling specificity (2009-2011), principal investigator.
Czech Science Foundation : Dynamics of proteins interacting with Dishevelled and their importance for Wnt signalling (2009-2013), principal investigator.

Supervision:
Emma Andersson (2004-2009) – PhD study, Karolinska Institutet, co-supervisor
Lukas Cajanek (from 2006) – PhD study, Karolinska Institutet, co-supervisor
Zuzana Spicarova (from 2006) – PhD study, Karolinska Institutet, external mentor
Carina Sandbäck (from 2008) – PhD study, Karolinska Institutet, co-supervisor
Michaela Kilander (from 2009) – PhD study, Karolinska Institutet, co-supervisor
Igor Cervenka (from 2008) – MSc. study, MasarykUniversity, Brno, supervisor
Jan Masek (from 2007) – MSc. study, MasarykUniversity, Brno, supervisor
Ranjani Sri Ganji (from 2008) – PhD study, MasarykUniversity, Brno, supervisor
Ondrej Bernatik (from 2008) – PhD study, MasarykUniversity, Brno, supervisor
Marketa Kaucka (from 2008) – PhD study, MasarykUniversity, Brno, supervisor

Ad hoc referee for:
Journal of Neurochemistry, Stem Cells, Journal of Cell Science, Journal of Experimental Medicine, Pharmacological Reviews, Grant Agency of the Czech Republic, Grant Agency of the Academy of Sciences of the Czech Republic

Selected publications
(out of 41 papers in peer reviewed journals):

1. Andersson ER, Bryjova L, Biris K, Yamaguchi TP, Arenas E*, Bryja V* (2010): Genetic interaction between Lrp6 and Wnt5a during mouse development. Dev Dyn. 2009 Sep 30. [Epub ahead of print]
2. Cajánek L, Ribeiro D, Liste I, Parish CL, Bryja V*, Arenas E* (2009): Wnt/beta-catenin signaling blockade promotes neuronal induction and dopaminergic differentiation in embryonic stem cells.Stem Cells. 2009 Sep 1. [Epub ahead of print]
3. Bryja V*, Andersson ER, Schambony A, Esner M, Bryjová L, Biris KK, Hall AC, Kraft B, Cajanek L, Yamaguchi TP, Buckingham M, Arenas E.* (2009): The Extracellular Domain of Lrp5/6 Inhibits Non-Canonical Wnt Signaling in vivo. Mol Biol Cell. 20: 924-936. Cover article.
4. V. Bryja¹, A. Schambony¹, L. Čajánek, I. Dominguez, E. Arenas and G. Schulte (2008): beta-Arrestin and casein kinase 1/2 define distinct branches of non-canonical WNT signalling pathways.EMBO Rep. 9(12): 1244-50. Featured article.
5. M. Andäng, J. Hjerling-Leffler, A. Moliner, T.K. Lundgren, G. Castelo-Branco, E. Nanou, E. Pozas, V. Bryja, S. Halliez, H. Nishimaru, J. Wilbertz, E. Arenas, M. Koltzenburg, M. Charnay, A. El Manira, C.F. Ibañez and P. Ernfors. Histone H2AX-dependent GABA_A receptor regulation of embryonic stem cell proliferation. Nature 451: 460-464, 2008
6. G. Schulte and V. Bryja. The Frizzled family of unconventional GPCRs. Trends Pharmacol. Sci. 28: 518-525, 2007.
7. V.Bryja, D. Gradl, A. Schambony, E. Arenas¹and G. Schulte¹. b-arrestin is a necessary component of Wnt/b-catenin signaling invitro and in vivo. Proc. Natl. Acad. Sci. USA 104: 6690-6695, 2007. .
8. V Bryja¹, G Schulte¹, N Rawal, A Grahn, and E Arenas. Wnt-5a induces Dishevelled phosphorylation and dopaminergic differentiation via a CK1δ/ε-dependent mechanism.J. Cell Sci. 120: 586-595, 2007.
9. V Bryja, S Bonillaand E Arenas. Derivation of mouse embryonic stem cells. Nature Protocols1: 2082-2087, 2006.
10. V Bryja¹, S Bonilla¹, L Čajánek, CL Parish, CM Schwartz, Y Luo, MS Rao and E Arenas. An efficient method for the derivation of mouse embryonic stem cells. Stem Cells 24: 844-849, 2006.

¹equal contribution, * corresponding author