Molecular Biophysics and Pharmacology
Colloidal polyaniline dispersions: Antibacterial activity, cytotoxicity and neutrophil oxidative burst
Published: COLLOIDS AND SURFACES B-BIOINTERFACES 116, 411-417 Authors: Kucekova, Z., Humpolicek, P., Kasparkova, V., Perecko, T., Lehocky, M., Hauerlandova, I., Saha, P., Stejskal, J. Year: 2014
Polyaniline colloids rank among promising application forms of this conducting polymer. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coil, with a minimum inhibitory concentration of 3500 mu g mL(-1). The data recorded on human keratinocyte (HaCaT) and a mouse embryonic fibroblast (NIH/3T3) cell lines using an MIT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with the absence of cytotoxic effect at around 150 mu g mL(-1). The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations <150 mu g mL(-1), was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed. (C) 2014 Elsevier B.V. All rights reserved.