c-Jun induces apoptosis of starved BM2 monoblasts by activating cyclin A-CDK2

Publikace: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 353, 92-97 Autoři: Vanhara, P., Bryja, V., Horvath, V., Kozubik, A., Hampl, A., Smarda, J. Rok: 2007

Abstrakt

c-Jun is one of the major components of the activating protein-1 (AP-1), the transcription factor that participates in regulation of proliferation, differentiation, and apoptosis. In this study, we explored functional interactions of the c-Jun protein with several regulators of the G1/S transition in serum-deprived v-myb-transformed chicken monoblasts BM2. We show that the c-Jun protein induces expression of cyclin A, thus up-regulating activity of cyclin A-associated cyclin-dependent kinase 2 (CDK2), and causing massive programmed cell death of starved BM2eJUN cells. Specific inhibition of CDK2 suppresses frequency of apoptosis of BM2cJUN cells. We conclude that up-regulation of cyclin A expression and CDK2 activity can represent important link between the c-Jun protein, cell cycle machinery, and programmed cell death pathway in leukemic cells. (c) 2006 Elsevier Inc. All rights reserved.