Biofyzikální chemie a molekulární onkologie
Voltammetric microanalysis of DNA adducts with osmium tetroxide,2,2 '-bipyridine using a pyrolytic graphite electrode
Publikace: TALANTA 56, 867-874 Autoři: Fojta, M., Havran, L., Kizek, R., Billova, S. Rok: 2002
DNA and synthetic polynucleotides modified with a complex of osmium tetroxide with 2,2'-bipyridine (Os,bipy) produce specific voltammetric signals at pyrolytic graphite electrodes. Based on a sufficient potential separation between the peaks of Os,bipy-modified DNA (DNA-Os,bipy) and of free Os,bipy, and using an adsorptive transfer stripping voltammetric procedure involving extraction of free Os,bipy from the electrode by chloroform, DNA-Os,bipy can be determined in an excess of the free reagent. Under certain conditions, 140 pg of DNA-Os,bipy can be detected after a 5 min accumulation period. This analysis displays a more favorable sensitivity and a better selectivity for DNA structure than oxidation of DNA guanine moieties, and offers detection of osmium DNA markers at carbon electrodes. (C) 2002 Elsevier Science B.V. All rights reserved.