Department of Free Radical Pathophysiology

The research program of the laboratory is focused on the study of reactive oxygen and nitrogen metabolites generated under physiological and pathophysiological conditions. The attention is aimed at human and animal phagocytes which constitute the front-line of the body?s defence mechanisms against invading microbial pathogens. Beside this beneficial role of neutrophils, high level production of neutrophil-derived reactive oxygen and nitrogen metabolites plays a role in a number of different diseases, such as inflammatory conditions that can ultimately lead to animal tissue injury.

The aims of the laboratory mainly involve the oxidative burst of phagocytes, interactions of phagocytes with endothelial cells, total antioxidant capacity as well as the contents of individual antioxidants in cells and body fluids, and the possibilities of oxidative injury prevention using drugs and dietary supplements. Highly qualified staff is skilled in luminometry, spectrophotometry, flow cytometry, hematology and other methods used in cellular biology. Beside the Academy of Sciences of the Czech Republic, the scientific program of the Laboratory is supported by several grants. An integral part of the Laboratory activities is also a collaboration with Czech and foreign institutes and universities. Results of the Laboratory have been summarized in several publications.

 

The main aims:
  • to develop exact chemiluminescence and flow cytometric analytical methods for the measurement of reactive oxygen and nitrogen metabolites generated by normal and transformed mammalian cells
  • to describe exactly the employment of antioxidants, nitric oxide donors/inhibitors, nonsteroidal anti-inflammatory drugs, and/or cytostatics for reactive oxygen and nitrogen metabolites-mediated modulation of metabolic activity, proliferation, differentiation and apoptosis of normal and transformed cell populations
  • to differentiate between extra- and intracellular radical generation
  • to study interactions of leukocytes with other cell populations (e.g. endothelial cells, platelets) under normal and pathophysiological conditions
  • to define the contribution of oxygen and nitrogen mediators to the pathobiology of ischemia-reperfusion injury (rat small intestine ischemia model, human open heart surgery, organ transplantation)