Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin

Časopis: BIOCHIMIE 95, 167-179
Autoři: Legartova, S., Jugova, A., Stixova, L., Kozubek, S., Fojtova, M., Zdrahal, Z., Lochmanova, G., Bartova, E.
Rok: 2013


Apoptotic bodies are the most condensed form of chromatin. In general, chromatin structure and function are mostly dictated by histone post-translational modifications. Thus, we have analyzed the histone signature in apoptotic cells, characterized by pronounced chromatin condensation. Here, H2B mono-acetylation, and H3K9 and H4 acetylation was significantly decreased in apoptotic cells, which maintained a high level of H3K9 methylation. This phenotype was independent of p53 function and distinct levels of anti-apoptotic Bcl2 protein. Interestingly, after etoposide treatment of leukemia and multiple myeloma cells, H3K9 and H4 hypoacetylation was accompanied by increased H3K9me2, but not H3K9me1 or H3K9me3. In adherent mouse fibroblasts, a high level of H3K9me3 and histone deacetylation in apoptotic bodies was likely responsible for the pronounced (similar to 40%) recovery of GFP-HP1 alpha and GFP-HP1 beta after photobleaching. HP1 mobility in apoptotic cells appeared to be unique because limited exchange after photobleaching was observed for other epigenetically important proteins, including GFP-JMJD2b histone demethylase (similar to 10% fluorescence recovery) or Polycomb group-related GFP-BMI1 protein (similar to 20% fluorescence recovery). These findings imply a novel fact that only certain subset of proteins in apoptotic bodies is dynamic. (c) 2012 Elsevier Masson SAS. All rights reserved.