Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin

Autoři: Sustackova, G., Kozubek, S., Stixova, L., Legartova, S., Matula, P., Orlova, D., Bartova, E.
Rok: 2012


Polycomb group (PcG) proteins, organized into Polycomb bodies, are important regulatory components of epigenetic processes involved in the heritable transcriptional repression of target genes. Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of the Polycomb group complex, PRC1. We used time-lapse confocal microscopy, micro-irradiation by UV laser (355nm) and GFP technology to study the dynamics and function of the BMI1 protein. We observed that BMI1 was recruited to UV-damaged chromatin simultaneously with decreased lysine acetylation, followed by the recruitment of heterochromatin protein HP1 beta to micro-irradiated regions. Pronounced recruitment of BMI1 was rapid, with half-time t=15sec; thus, BMI1 is likely involved in the initiation step leading to the recognition of UV-damaged sites. Histone hyperacetylation, stimulated by HDAC inhibitor TSA, suppression of transcription by actinomycin D, and ATP-depletion prevented increased accumulation of BMI1 to H2AX-positive irradiated chromatin. Moreover, BMI1 had slight ability to recognize spontaneously occurring DNA breaks caused by other pathophysiological processes. Taken together, our data indicate that the dynamics of recognition of UV-damaged chromatin, and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the recruitment of HP beta to UV-irradiated chromatin. J. Cell. Physiol. 227: 18381850, 2012. (C) 2011 Wiley Periodicals, Inc.