Mapping of interaction domains of putative telomere-binding proteins AtTRB1 and AtPOT1b from Arabidopsis thaliana

Časopis: FEBS LETTERS 582, 1400-1406
Autoři: Schrumpfova, PP., Kuchar, M., Palecek, J., Fajkus, J.
Rok: 2008

Abstrakt

We previously searched for interactions between plant telomere-binding proteins and found that AtTRB1, from the single-myb-histone (Smh) family, interacts with the Arabidopsis POT1-like-protein, AtPOT1b, involved in telomere capping. Here we identify domains responsible for that interaction. We also map domains in AtTRB1 responsible for interactions with other Smh-family-members. Our results show that the N-terminal OB-fold-domain of AtPOT1b mediates the interaction with AtTRB1. This domain is characteristic for POT1-proteins and is involved with binding the G-rich-strand of telomeric DNA. AtPOT1b also interacts with AtTRB2 and AtTRB3. The central histone-globular-domain of AtTRB1 is involved with binding to AtTRB2 and 3, as well as to AtPOT1b. AtTRB1-heterodimers with other Smh-family-members are more stable than AtTRB1-homodimers. Our results reveal interaction networks of plant telomeres. Structured summary: MINT-6440051: AtTRB1 (uniprotkb: Q8VWK4) physically interacts (MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by two-hybrid ( MI: 0018) MINT-6440068: AtTRB2 ( uniprotkb: Q8VX38) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by two-hybrid ( MI: 0018) MINT-6440083: AtTRB3 ( uniprotkb: Q9M2X3) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by two-hybrid ( MI: 0018) MINT-6440099: AtPOT1b ( uniprotkb: Q6Q835) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by two-hybrid ( MI: 0018) MINT-6440119: AtPOT1b ( uniprotkb: Q6Q835) physically interacts ( MI: 0218) with AtTRB2 ( uniprotkb: Q8VX38) by two-hybrid ( MI: 0018) MINT-6440138: AtPOT1b ( uniprotkb: Q6Q835) physically interacts ( MI: 0218) with AtTRB3 ( uniprotkb: Q9M2X3) by two-hybrid ( MI: 0018) MINT-6440216: AtPOT1b ( uniprotkb: Q6Q835) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by coimmunoprecipitation ( MI: 0019) MINT-6440157: AtTRB2 ( uniprotkb: Q8VX38) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by coimmunoprecipitation ( MI: 0019) MINT-6440177: AtTRB3 ( uniprotkb: Q9M2X3) physically interacts ( MI: 0218) with AtTRB1 ( uniprotkb: Q8VWK4) by coimmunoprecipitation ( MI: 0019) (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.