Detecting DNA damage and monitoring of enzymatic DNA processing by electrochemical methods

Electrochemical studies of DNA damage belong to traditionally successful topics of the IBP bioelectrochemistry school. Recently the electrochemical approaches were combined with enzymatic DNA cleavage to propose techniques of sensitive detection of specific damage to nucleobases, and to elaborate techniques for rapid screening of the activities of nucleic acids processing enzymes such as nucleases or DNA ligases. Proof-of-principle results were published in prestigious Analytical Chemistry journal and promising collaboration with a UK partner was established to focus on further application of electrochemistry in studies of enzymatic activities of recombinant protein constructs.

K. Cahova-Kucharikova, M. Fojta, T. Mozga, and E. Palecek, Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells. Analytical Chemistry 77, 2920-2927, 2005.

J. Vacek, K. Cahova, E. Palecek, D.R. Bullard, M. Lavesa-Curto, R.P. Bowater, and M. Fojta, Label-free electrochemical monitoring of DNA ligase activity. Analytical Chemistry 80, 7609-7613, 2008.