Sensitive and specific analysis of purine nucleobases, metabolites and drugs with mechanically roughened carbon electrodes

This work resulted in a considerable improvement of voltammetric determination of purine derivatives, metabolites and analogues used as drugs by their direct electrochemical oxidation. At mechanically roughened (“grinded”) carbon electrodes (in contrast to other types of electrodes), excellent separation of oxidation peaks of uric acid, xanthine, hypoxanthine, adenine, guanine, allopurinol and oxypurinol from each other and from signals of interferents naturally occurring in biological matrices (such as urine), was attained. This allowed highly selective detection of these species in mixtures, monitoring of their consecutive enzymatic conversions as well as determination in clinical samples. These proposed electrochemical approaches are clearly superior to other, in general rather time consuming and more expensive, analytical techniques

S. Hason, S. Stepankova, A. Kourilova, V. Vetterl, J. Lata, M. Fojta, and F. Jelen, Simultaneous Electrochemical Monitoring of Metabolites Related to the Xanthine Oxidase Pathway Using a Grinded Carbon Electrode. Analytical Chemistry 81, 4302-4307, 2009.

S. Hason, V. Vetterl, F. Jelen, and M. Fojta, Improved sensitivity and selectivity of uric acid voltammetric sensing with mechanically grinded carbon/graphite electrodes. Electrochimica Acta 54, 1864-1873, 2009.

S. Hason, L. Fojt, P. Sebest, and M. Fojta, Improved Electrochemical Detection of Purine Nucleobases at Mechanically Roughened Edge-Plane Pyrolytic Graphite Electrode. Electroanalysis 21, 666-670, 2009.