Novel strategies of labeled nucleic acids construction and their application in electrochemical DNA sensing

A fruitful collaboration with the group of M. Hocek at the IOCB ASCR in Prague resulted in development of novel facile techniques of enzymatic construction of modified, functionalized and labeled nucleic acids using modified deoxynucleotide triphosphates (the synthesis of which has been developed in the Hocek lab). Particularly in the area of electrochemical DNA sensing, including DNA minisequencing/SNP typing based on “multicolor” electrochemical DNA coding, promising results were obtained and published in prestigious journals. These papers have gained considerable number of citations within relatively short time.

P. Brazdilova, M. Vrabel, R. Pohl, H. Pivonkova, L. Havran, M. Hocek, and M. Fojta, Ferrocenylethynyl derivatives of nucleoside triphosphates: Synthesis, incorporation, electrochemistry, and bioanalytical applications. Chemistry-a European Journal 13,

9527-9533, 2007.

H. Cahova, L. Havran, P. Brazdilova, H. Pivonkova, R. Pohl, M. Fojta, and M. Hocek, Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates: Synthesis, enzymatic incorporation, and electrochemical detection. Angewandte Chemie-International Edition 47, 2059-2062, 2008.

M. Vrabel, P. Horakova, H. Pivonkova, L. Kalachova, H. Cernocka, H. Cahova, R. Pohl, P. Sebest, L. Havran, M. Hocek, and M. Fojta, Base-Modified DNA Labeled by [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) Complexes: Construction by Polymerase Incorporation of Modified Nucleoside Triphosphates, Electrochemical and Luminescent Properties, and Applications. Chemistry-a European Journal 15, 1144-1154, 2009.